Bromodeoxyuridine 5'-monophosphate incorporation into yeast nuclear and mitochondrial deoxyribonucleic acid

Quantitative studies of incorporation of exogenous thymidine and 5-bromodeoxyuridine into deoxyribonucleic acid of mammalian cells in vitro.

Methods for the synthesis of bromodeoxyuridine-Br s2 and bromodeoxyuridine-H a (BUdR) are described. The incorporation of both thymidine and BUdR into the deoxyribonucleic acid (DNA) of rat bone marrow cells in vitro was markedly influenced by concentrations of thymidine and BUdR, respectively, in the incubation media. At equivalent concentrations of respective substrate, the amount of thymidin...

The incorporation of nucleotides into amino acid transfer ribonucleic acid. II. Evidence for separate enzymatic sites for incorporation of adenosine 5'-monophosphate and cytidine 5'-monophosphate.

The preceding paper described the purification and some of the properties of an enzyme present in rabbit muscle extracts that catalyzes the incorporation of adenylic acid from adenosine triphosphate into the terminal position of amino acid transfer ribonucleic acid obtained from yeast (1). The data presented in this paper are concerned with the incorporation of cytidine 5’-monophosphate into th...

Enzymatic Synthesis of Deoxyribonucleic Acid. Iii. the Incorporation of Pyrimidine and Purine Analogues into Deoxyribonucleic Acid.

Equal incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid.

After infection of Escherichia coli B with radiolabeled T7 bacteriophage, the parental deoxyribonucleic acid label was found in both polynucleotide chains of the intracellular T7 concatemer.

Thymidine incorporation into deoxyribonucleic acid by isolated rat liver mitochondria.

Isolated rat liver mitochondria can incorporate tritiated thymidine into DNA. The addition of deoxyribonucleotides and ATP are necessary. The process of incorporation was found to be inhibited to the extent of 92% by actinomycin D and only 14% by pancreatic deoxyribonuclease. The identification of the labeled product as mitochondrial DNA is supported by its insolubility in acid, resistance to a...